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voltage dependent anion channel vdac  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc voltage dependent anion channel vdac
    Voltage Dependent Anion Channel Vdac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/voltage+dependent+anion+channel+vdac/pm41597199-118-42-47
    Average 86 stars, based on 1 article reviews
    voltage dependent anion channel vdac - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Lactate Contributes to Remote Ischemic Preconditioning-Mediated Protection Against Myocardial Ischemia Reperfusion Injury by Facilitating Autophagy via the AMP-Activated Protein Kinase-Mammalian Target of Rapamycin-Transcription Factor EB-Connexin 43 Axis.
    Article Snippet: Address correspondence to LiPing Jiang, Ph.D., Jiangxi Provincial Key Laboratory of Drug Targets and Drug Screening, School of Pharmacy, Jiangxi Medical College, Nanchang University, 461 Bayi Ave., Nanchang, Jiangxi 330006, China; or Xiao-Jian Han, Ph.D., Institute of Geriatrics, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, 152 Aiguo Rd., Nanchang, Jiangxi 330006, China.. E-mail: lpjiang@ncu.edu.cn or hanxiaojian@hotmail.com.

    Article Title: Estrogen-Induced Hypermethylation Silencing of RPS2 and TMEM177 Inhibits Energy Metabolism and Reduces the Survival of CRC Cells.
    Article Snippet: .. The membranes were incubated overnight at 4 ◦C with primary antibodies against Cytochrome c (CYCS), cytochrome C oxidase subunit IV (COX IV), heat shock protein 60 (HSP60), prohibitin 1 (PHB1), pyruvate dehydrogenase (PDH), succinate dehydrogenase subunit A (SDHA), superoxide dismutase 1 (SOD1), voltage-dependent anion channel (VDAC) (CAT#8674, Cell Signaling Technology, Danvers, MA, USA), ERα and ERβ (Ab75635 and ab288, Abcam, Cambridge, UK), RPS2 and TMEM177 (NBP2-14852 and NBP2-31567, R&D Systems, Minneapolis, MN, USA), β-actin (CAT#4967s, Cell Signaling Technology, Danvers, MA, USA). ..

    Article Title: Melatonin Reduces Aggravation of Renal Ischemia–Reperfusion Injury in Obese Rats by Maintaining Mitochondrial Homeostasis and Integrity through AMPK/PGC-1α/SIRT3/SOD2 Activation
    Article Snippet: Aliquots of protein lysates were separated on 10% SDS-PAGE gels, blotted onto nitrocellulose membranes (Millipore, Burlington, MA, USA), and blocked with 5% skim milk or bovine serum albumin in 0.1% Tris-buffered saline and Tween (TBST). .. The membranes were incubated at 4 °C overnight with primary antibodies against AMP-activated protein kinase (AMPK), sirtuin 3 (SIRT3), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl-2), pro-caspase3, cleaved-caspase3, dynamin-like protein 1 (Drp1), phospho-dynamin-like protein 1 at Ser616 (p-Drp1Ser616), mitofusin 2 (Mfn2), PTEN-induced putative kinase 1 (PINK1), voltage-dependent anion channel (VDAC), PARKIN, β-actin (Cell Signaling Technology, Danvers, MA, USA), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), phospho-AMPK at Thr172 (p-AMPKThr172) (Millipore Corporation, Burlington, MA, USA), acetylated SOD2 (Ac-SOD2), and Bcl-2-associated X protein (Bax) (Abcam, Cambridge, MA, USA), followed by the corresponding secondary antibodies. .. Protein bands were detected using the ChemiDoc Touch Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and densitometric analysis was performed by the ImageJ program (National Institute of Health, Rockville, MD, USA).

    Article Title: Melatonin Reduces Aggravation of Renal Ischemia-Reperfusion Injury in Obese Rats by Maintaining Mitochondrial Homeostasis and Integrity through AMPK/PGC-1α/SIRT3/SOD2 Activation.
    Article Snippet: Aliquots of protein lysates were separated on 10% SDS-PAGE gels, blotted onto nitrocellulose membranes (Millipore, Burlington, MA, USA), and blocked with 5% skim milk or bovine serum albumin in 0.1% Tris-buffered saline and Tween (TBST). .. The membranes were incubated at 4 ◦C overnight with primary antibodies against AMP-activated protein kinase (AMPK), sirtuin 3 (SIRT3), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl-2), pro-caspase3, cleaved-caspase3, dynamin-like protein 1 (Drp1), phospho-dynamin-like protein 1 at Ser616 (p-Drp1Ser616), mitofusin 2 (Mfn2), PTENinduced putative kinase 1 (PINK1), voltage-dependent anion channel (VDAC), PARKIN, β-actin (Cell Signaling Technology, Danvers, MA, USA), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), phospho-AMPK at Thr172 (p-AMPKThr172) (Millipore Corporation, Burlington, MA, USA), acetylated SOD2 (Ac-SOD2), and Bcl-2associated X protein (Bax) (Abcam, Cambridge, MA, USA), followed by the corresponding secondary antibodies. .. Protein bands were detected using the ChemiDoc Touch Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and densitometric analysis was performed by the ImageJ program (National Institute of Health, Rockville, MD, USA).

    Article Title: Calpain inhibition decreases oxidative stress via mitochondrial regulation in a swine model of chronic myocardial ischemia.
    Article Snippet: Introduction: Calpain overexpression is implicated in mitochondrial damage leading to tissue oxidative stress and myocardial ischemic injury.. The aim of this study was to determine the effects of calpain inhibition (CI) on mitochondrial impairment and oxidative stress in a swine model of chronic myocardial ischemia and metabolic

    Article Title: Estrogen-Induced Hypermethylation Silencing of RPS2 and TMEM177 Inhibits Energy Metabolism and Reduces the Survival of CRC Cells
    Article Snippet: .. The membranes were incubated overnight at 4 °C with primary antibodies against Cytochrome c (CYCS), cytochrome C oxidase subunit IV (COX IV), heat shock protein 60 (HSP60), prohibitin 1 (PHB1), pyruvate dehydrogenase (PDH), succinate dehydrogenase subunit A (SDHA), superoxide dismutase 1 (SOD1), voltage-dependent anion channel (VDAC) (CAT#8674, Cell Signaling Technology, Danvers, MA, USA), ERα and ERβ (Ab75635 and ab288, Abcam, Cambridge, UK), RPS2 and TMEM177 (NBP2-14852 and NBP2-31567, R&D Systems, Minneapolis, MN, USA), β-actin (CAT#4967s, Cell Signaling Technology, Danvers, MA, USA). ..

    Pyrolysis Gas Chromatography:

    Article Title: Melatonin Reduces Aggravation of Renal Ischemia–Reperfusion Injury in Obese Rats by Maintaining Mitochondrial Homeostasis and Integrity through AMPK/PGC-1α/SIRT3/SOD2 Activation
    Article Snippet: Aliquots of protein lysates were separated on 10% SDS-PAGE gels, blotted onto nitrocellulose membranes (Millipore, Burlington, MA, USA), and blocked with 5% skim milk or bovine serum albumin in 0.1% Tris-buffered saline and Tween (TBST). .. The membranes were incubated at 4 °C overnight with primary antibodies against AMP-activated protein kinase (AMPK), sirtuin 3 (SIRT3), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl-2), pro-caspase3, cleaved-caspase3, dynamin-like protein 1 (Drp1), phospho-dynamin-like protein 1 at Ser616 (p-Drp1Ser616), mitofusin 2 (Mfn2), PTEN-induced putative kinase 1 (PINK1), voltage-dependent anion channel (VDAC), PARKIN, β-actin (Cell Signaling Technology, Danvers, MA, USA), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), phospho-AMPK at Thr172 (p-AMPKThr172) (Millipore Corporation, Burlington, MA, USA), acetylated SOD2 (Ac-SOD2), and Bcl-2-associated X protein (Bax) (Abcam, Cambridge, MA, USA), followed by the corresponding secondary antibodies. .. Protein bands were detected using the ChemiDoc Touch Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and densitometric analysis was performed by the ImageJ program (National Institute of Health, Rockville, MD, USA).

    Article Title: Melatonin Reduces Aggravation of Renal Ischemia-Reperfusion Injury in Obese Rats by Maintaining Mitochondrial Homeostasis and Integrity through AMPK/PGC-1α/SIRT3/SOD2 Activation.
    Article Snippet: Aliquots of protein lysates were separated on 10% SDS-PAGE gels, blotted onto nitrocellulose membranes (Millipore, Burlington, MA, USA), and blocked with 5% skim milk or bovine serum albumin in 0.1% Tris-buffered saline and Tween (TBST). .. The membranes were incubated at 4 ◦C overnight with primary antibodies against AMP-activated protein kinase (AMPK), sirtuin 3 (SIRT3), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl-2), pro-caspase3, cleaved-caspase3, dynamin-like protein 1 (Drp1), phospho-dynamin-like protein 1 at Ser616 (p-Drp1Ser616), mitofusin 2 (Mfn2), PTENinduced putative kinase 1 (PINK1), voltage-dependent anion channel (VDAC), PARKIN, β-actin (Cell Signaling Technology, Danvers, MA, USA), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), phospho-AMPK at Thr172 (p-AMPKThr172) (Millipore Corporation, Burlington, MA, USA), acetylated SOD2 (Ac-SOD2), and Bcl-2associated X protein (Bax) (Abcam, Cambridge, MA, USA), followed by the corresponding secondary antibodies. .. Protein bands were detected using the ChemiDoc Touch Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and densitometric analysis was performed by the ImageJ program (National Institute of Health, Rockville, MD, USA).

    Membrane:

    Article Title: Calpain inhibition decreases oxidative stress via mitochondrial regulation in a swine model of chronic myocardial ischemia.
    Article Snippet: Introduction: Calpain overexpression is implicated in mitochondrial damage leading to tissue oxidative stress and myocardial ischemic injury.. The aim of this study was to determine the effects of calpain inhibition (CI) on mitochondrial impairment and oxidative stress in a swine model of chronic myocardial ischemia and metabolic



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    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), <t>VDAC</t> ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.
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    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), <t>VDAC</t> ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.
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    Image Search Results


    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.

    Journal: Antioxidants

    Article Title: Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction

    doi: 10.3390/antiox14101219

    Figure Lengend Snippet: Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.

    Article Snippet: Subsequently, they were incubated with primary antibodies diluted in 0.1% bovine serum albumin (BSA), against alpha-smooth muscle actin (α-SMA) 1:1000 (ab5694, Rb, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA) 1:1000 (13110, Rb, Cell Signaling, Boston, MA, USA), Acetyl-Histone H3 (Lys9) 1:1000 (9649, Rb, Cell Signaling, Boston, MA, USA), beclin-1 1:1000 (3495, Rb, Cell Signaling, Boston, MA, USA), Sequestosome-1 (SQSTM1)/p62 1:1000 (5114, Rb, Cell Signaling, Boston, MA, USA), complex IV 1:1000 (ab109863, Ms, Abcam, Cambridge, UK), complex I 1:1000 (A21344, Ms, Thermofisher Scientific, Carlsbad, CA, USA), cytochrome c (cyt c) 1:1000 (556433, Ms, BD Biosciences, San Jose, CA, USA), ATP synthase subunit b 1:1000 (MABS1304, Ms, Millipore, St. Louis, MO, USA), voltage-dependent anion-selective channel (VDAC) 1:1000 (4866, Rb, Cell Signaling, Boston, MA, USA), and β-actin 1:2000 (A2228, Ms, Sigma-Aldrich, St. Louis, MO, USA) at 4 °C overnight.

    Techniques:

    Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).

    Journal: Antioxidants

    Article Title: Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction

    doi: 10.3390/antiox14101219

    Figure Lengend Snippet: Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).

    Article Snippet: Subsequently, they were incubated with primary antibodies diluted in 0.1% bovine serum albumin (BSA), against alpha-smooth muscle actin (α-SMA) 1:1000 (ab5694, Rb, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA) 1:1000 (13110, Rb, Cell Signaling, Boston, MA, USA), Acetyl-Histone H3 (Lys9) 1:1000 (9649, Rb, Cell Signaling, Boston, MA, USA), beclin-1 1:1000 (3495, Rb, Cell Signaling, Boston, MA, USA), Sequestosome-1 (SQSTM1)/p62 1:1000 (5114, Rb, Cell Signaling, Boston, MA, USA), complex IV 1:1000 (ab109863, Ms, Abcam, Cambridge, UK), complex I 1:1000 (A21344, Ms, Thermofisher Scientific, Carlsbad, CA, USA), cytochrome c (cyt c) 1:1000 (556433, Ms, BD Biosciences, San Jose, CA, USA), ATP synthase subunit b 1:1000 (MABS1304, Ms, Millipore, St. Louis, MO, USA), voltage-dependent anion-selective channel (VDAC) 1:1000 (4866, Rb, Cell Signaling, Boston, MA, USA), and β-actin 1:2000 (A2228, Ms, Sigma-Aldrich, St. Louis, MO, USA) at 4 °C overnight.

    Techniques: Comparison